SELECTIVE "STAINING" FOR ELECTRON MICROGRAPHY
نویسندگان
چکیده
منابع مشابه
Selective "staining" for Electron Micrography
The physical basis of contrast and image formation in electron micrography is considered in relation to the possibility of recording selective chemical effects on cell components. A technology of selective microchemical analysis, equivalent to differential staining, is suggested as practicable in electron micrography. Electron pictures of bacteria after exposure to salts of heavy metals have sh...
متن کاملSection Staining for Electron Microscopy
Epon epoxy resin was introduced as an embedding medium for biological electron microscopy largely through the independent efforts of Finck (1) and Luft (2). The usual recipe involves three components: Epon 812 resin (Epikote 812, in England), an anhydride hardener, and an accelerator such as DMP-30. Since the combination of Epon with dodecenyl succinic anhydride (DDSA) was thought to produce a ...
متن کاملElectron Micrography of Bud Formation in Metschnikowia krissii.
The fine structure of bud formation of Metschnikowia krissii was studied by means of ultramicrotomy and transmission electron microscopy. Bud protrusion and development were observed by scanning electron microscopy. Bud formation in this yeast takes place by an extension of a small localized area of the existing parent wall. The parent cell and its bud are initially separated by the plasmalemma...
متن کاملAn Improved Staining Method for Electron Microscopy
A n a q u e o u s so lu t ion of u r a n y l ace t a t e was first e m p l o y e d as a n e l ec t ron s ta in by W a t s o n (1958). W e h a v e f o u n d t h a t this s ta in , a l t h o u g h f r e q u e n t l y effective, is capr ic ious . T h e s ta in m a y no t inc rease con t r a s t of a g iven spec imen , a n d m a y , in o the r ins tances , l eave u n d e s i r a b l e c o n t a m i...
متن کاملBalbiani ring hnRNP substructure visualized by selective staining and electron spectroscopic imaging
The Balbiani Rings (BR) in the polytene chromosomes of Chironomus salivary glands are intense sites of transcription. The nascent RNPs fold during transcription into 40-50-nm granules, containing in the mature transcript approximately 37-kb RNA. Using a new nucleic acid specific stain, osmium ammine B on Lowicryl sections, in combination with electron energy filtered imaging of sections contain...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1942
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.76.1.103